Picazarri Karina, Luna-Arias Juan Pedro, Carrillo Eduardo, Orozco Esther, Rodriguez Mario A
Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN, A.P. 14-740, Mexico, D.F. 07000, Mexico.
Exp Parasitol. 2005 Jul;110(3):253-8. doi: 10.1016/j.exppara.2005.02.014. Epub 2005 Apr 1.
EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment that encodes the C-terminus of EhGPCR-1. The cloning and sequence of the full-length cDNA revealed that it predicts a polypeptide with two tyrosine-based sorting signals for endocytosis and seven transmembranal domains. These results suggest that EhGPCR-1 could be a GPCR involved in phagocytosis. EhGPCR-1 could be a member of the Rhodopsin family, characterized by a short N-terminus without cysteine residues.
EhRabB是一种参与吞噬作用的溶组织内阿米巴蛋白。然而，调节EhRabB活性的蛋白质尚不清楚。在此，我们报告鉴定出一种与EhRabB结合的溶组织内阿米巴假定G蛋白偶联受体（EhGPCR-1）。通过双杂交筛选，我们发现了一个编码EhGPCR-1 C末端的372 bp cDNA片段。全长cDNA的克隆和序列分析表明，它预测的多肽具有两个基于酪氨酸的内吞分选信号和七个跨膜结构域。这些结果表明EhGPCR-1可能是一种参与吞噬作用的GPCR。EhGPCR-1可能是视紫红质家族的成员，其特征是N末端较短且无半胱氨酸残基。
