Guzmán-Medrano Rebeca, Castillo-Juárez Beatriz A, García-Pérez Rosa María, Salas-Casas Andrés, Orozco Esther, Rodríguez Mario A
Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN. A.P. 14-740, Mexico, D.F. 07000, Mexico.
Exp Parasitol. 2005 Jul;110(3):259-64. doi: 10.1016/j.exppara.2005.03.002. Epub 2005 Apr 9.
We analyzed the expression and location of EhRabB in clone L-6, a phagocytosis-deficient mutant of Entamoeba histolytica, in comparison with the wild-type clone A. Intriguingly, trophozoites of clone L-6 express more EhRabB than those of clone A. However, the majority of EhRabB-containing vesicles remained in the cytoplasm of clone L-6 during phagocytosis. To investigate molecular alterations in EhRabB of clone L-6 we compared the EhrabB gene sequences from clones L-6 and A. We also isolated, sequenced and compared the RabB protein of Entamoeba dispar. Results showed that EhrabB gene of clone L-6 is 98.2 and 94.1% identical to rabB genes of E. dispar and clone A, respectively. The rabB genes from clone A and E. dispar have 92.2% identity. Four out of five amino acids changes in RabB proteins of clone L-6 and E. dispar are shared. These changes may alter the binding of effector proteins and the specific subcellular location of EhRabB.
我们分析了溶组织内阿米巴吞噬缺陷型突变体克隆L-6中EhRabB的表达和定位，并与野生型克隆A进行了比较。有趣的是，克隆L-6的滋养体比克隆A的滋养体表达更多的EhRabB。然而，在吞噬过程中，大多数含有EhRabB的囊泡留在克隆L-6的细胞质中。为了研究克隆L-6的EhRabB的分子变化，我们比较了克隆L-6和A的EhrabB基因序列。我们还分离、测序并比较了迪斯帕内阿米巴的RabB蛋白。结果表明，克隆L-6的EhrabB基因与迪斯帕内阿米巴和克隆A的rabB基因分别有98.2%和94.1%的同一性。克隆A和迪斯帕内阿米巴的rabB基因有92.2%的同一性。克隆L-6和迪斯帕内阿米巴的RabB蛋白的五个氨基酸变化中有四个是相同的。这些变化可能会改变效应蛋白的结合以及EhRabB的特定亚细胞定位。
