Tanyuksel Mehmet, Yilmaz Hasan, Ulukanligil Mustafa, Araz Engin, Cicek Mutalip, Koru Ozgur, Tas Zeynep, Petri William A
Division of Medical Parasitology, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Etlik 06018, Ankara, Turkey.
Exp Parasitol. 2005 Jul;110(3):322-6. doi: 10.1016/j.exppara.2005.02.012. Epub 2005 Mar 23.
Diagnosis of amebiasis is usually performed on a clinical basis alone in most endemic countries having limited economic resources. This epidemiological study was conducted using modern diagnostic tests for amebiasis in the southeastern region of Turkey, an endemic area for amebiasis. The population of this study included patients with symptomatic diarrhea/dysentery attending both Yuzuncu Yil University, Van and Harran University, Sanliurfa, Turkey. A total of 380 stool specimens were collected and examined for Entamoeba by light microscopy (fresh, lugol, and trichrome staining) and stool antigen detection based- enzyme-linked immunosorbent assay (EIA) test (TechLab Entamoeba histolytica II). 24% (91/380) of stool specimens were positive for E. histolytica/Entamoeba dispar trophozoites/cysts microscopically using trichrome staining. 13% (51/380) of the stool specimens were found to be positive for E. histolytica by the EIA test, including 15% (14/91) of microscopy (+) stool specimens and 13% (37/289) of microscopy (-) stool specimens. Enteric parasites were common in these populations with 66% (251/380) of the study population harboring more than one parasite. In addition to the 13% (51/380) of patients determined to have E. histolytica by EIA, eighty-six patients (22.6%) had Blastocystis hominis, 54 (14.2%) Entamoeba coli, 44 (11.5%) Giardia lamblia, 16 (4.2%) Chilomastix mesnili, 15 (3.9%) Iodamoeba bütschlii, 12 (3.1%) Hymenolepis nana, 9 (2.3%) Endolimax nana, 9 (2.3%) Dientamoeba fragilis, and 8 (2.1%) had Ascaris lumbricoides. We concluded that E. histolytica infection was found in 13% of the patients presenting with diarrhea in Van and Sanliurfa Turkey.
在大多数经济资源有限的流行国家，阿米巴病的诊断通常仅基于临床诊断。本流行病学研究在土耳其东南部地区（阿米巴病的流行区）使用现代诊断测试对阿米巴病进行了调查。该研究的人群包括土耳其凡城的尤祖恩居尔大学和舍尔纳克省的哈兰大学出现症状性腹泻/痢疾的患者。共收集了380份粪便标本，通过光学显微镜（新鲜标本、卢戈氏碘液染色和三色染色）以及基于粪便抗原检测的酶联免疫吸附测定（EIA）试验（TechLab溶组织内阿米巴II）对溶组织内阿米巴进行检测。使用三色染色法在显微镜下观察，24%（91/380）的粪便标本中溶组织内阿米巴/迪斯帕内阿米巴滋养体/包囊呈阳性。通过EIA试验发现13%（51/380）的粪便标本中溶组织内阿米巴呈阳性，其中包括15%（14/91）显微镜检查阳性的粪便标本和13%（37/289）显微镜检查阴性的粪便标本。肠道寄生虫在这些人群中很常见，66%（251/380）的研究人群感染了不止一种寄生虫。除了通过EIA确定有13%（51/380）的患者感染溶组织内阿米巴外，86名患者（22.6%）感染人芽囊原虫，54名（14.2%）感染结肠内阿米巴，44名（11.5%）感染蓝氏贾第鞭毛虫，16名（4.2%）感染梅氏唇鞭毛虫，15名（3.9%）感染布氏嗜碘阿米巴，12名（3.1%）感染微小膜壳绦虫，9名（2.3%）感染内蜒阿米巴，9名（2.3%）感染脆弱双核阿米巴，8名（2.1%）感染蛔虫。我们得出结论，在土耳其凡城和舍尔纳克省出现腹泻的患者中，13%的患者感染了溶组织内阿米巴。
