Takeuchi Mio, Nanba Kenji, Iwamoto Hiroshi, Nirei Hisashi, Kusuda Takashi, Kazaoka Osamu, Owaki Masato, Furuya Ken
Department of Aquatic Bioscience, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
Water Res. 2005 Jun;39(11):2438-44. doi: 10.1016/j.watres.2005.04.041.
At a trichloroethylene (TCE)-contaminated site in Chikura, Chiba, Japan, TCE had spread over to the first and second aquifers over years. After 8 years of pumping and treatment, finally derivative of TCE, cis-dichloroethylene (c-DCE) remained only in the second aquifer. In this study, feasibility of a low cost in situ bioremediation utilizing groundwater of the third aquifer, which contained natural dissolved methane possibly derived from natural gas field nearby, to stimulate methane-oxidizing bacteria was examined. In vitro experiment showed that a mixture of the groundwater from the second and third aquifers stimulated a growth of methane oxidizing bacteria and enhanced c-DCE degradation. The groundwater of the third aquifer was introduced into the second aquifer in situ. The population of methanotrophs with high V(max) and K(m) for methane uptake increased, resulting in successful degradation of c-DCE at a monitoring well 2m downgradient of the injection well.
在日本千叶县千叶市一个受三氯乙烯（TCE）污染的场地，多年来TCE已扩散到第一和第二含水层。经过8年的抽水和处理，最终TCE的衍生物顺式二氯乙烯（c-DCE）仅残留在第二含水层中。在本研究中，考察了利用第三含水层的地下水进行低成本原位生物修复的可行性，该含水层含有可能源自附近天然气田的天然溶解甲烷，以刺激甲烷氧化细菌生长。体外实验表明，第二和第三含水层的地下水混合物刺激了甲烷氧化细菌的生长并增强了c-DCE的降解。将第三含水层的地下水原位引入第二含水层。对甲烷吸收具有高V(max)和K(m)的甲烷营养菌数量增加，导致在注入井下游2米处的监测井中c-DCE成功降解。
