Institute of Chemical Technology Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Technicka 3, 166 28 Prague, Czech Republic.
Biotechnol Adv. 2013 Mar-Apr;31(2):154-65. doi: 10.1016/j.biotechadv.2012.09.003. Epub 2012 Sep 26.
Microbial biodegradation and biotransformation reactions are essential to most bioremediation processes, yet the specific organisms, genes, and mechanisms involved are often not well understood. Stable isotope probing (SIP) enables researchers to directly link microbial metabolic capability to phylogenetic and metagenomic information within a community context by tracking isotopically labeled substances into phylogenetically and functionally informative biomarkers. SIP is thus applicable as a tool for the identification of active members of the microbial community and associated genes integral to the community functional potential, such as biodegradative processes. The rapid evolution of SIP over the last decade and integration with metagenomics provide researchers with a much deeper insight into potential biodegradative genes, processes, and applications, thereby enabling an improved mechanistic understanding that can facilitate advances in the field of bioremediation.
微生物的生物降解和生物转化反应对大多数生物修复过程至关重要，但涉及的特定生物、基因和机制通常并不清楚。稳定同位素示踪 (SIP) 通过将同位素标记的物质追踪到具有系统发育和功能信息的生物标志物中，使研究人员能够在群落背景下直接将微生物代谢能力与系统发育和宏基因组信息联系起来。因此，SIP 可作为一种工具，用于鉴定微生物群落中的活性成员和与群落功能潜力相关的基因，如生物降解过程。在过去十年中，SIP 的快速发展及其与宏基因组学的结合为研究人员提供了对潜在生物降解基因、过程和应用的更深入了解，从而使人们对生物修复领域的机制有了更好的理解，从而推动了该领域的发展。
