Somerville Wendy, Thibert Louise, Schwartzman Kevin, Behr Marcel A
McGill University Health Centre Research Institute, Montreal, Quebec, Canada.
J Clin Microbiol. 2005 Jun;43(6):2996-7. doi: 10.1128/JCM.43.6.2996-2997.2005.
DNA fingerprinting of Mycobacterium tuberculosis by IS6110 restriction fragment length polymorphism analysis requires substantial high-quality DNA. We demonstrated that, despite extraction treatments that might be expected to inactivate this organism, M. tuberculosis remained viable during this process. These data suggest that the extraction of M. tuberculosis DNA should be performed within containment until complete.
通过IS6110限制性片段长度多态性分析对结核分枝杆菌进行DNA指纹图谱分析需要大量高质量的DNA。我们证明,尽管采用了可能会使该生物体失活的提取处理方法,但结核分枝杆菌在此过程中仍保持存活。这些数据表明,结核分枝杆菌DNA的提取应在隔离环境中进行直至完成。