Agrawal Ganesh Kumar, Rakwal Randeep
Research Laboratory for Agricultural Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal.
Mass Spectrom Rev. 2006 Jan-Feb;25(1):1-53. doi: 10.1002/mas.20056.
Proteomics-a systematic study of proteins present in a cell, tissue, organ, or organism at a particular moment during the life cycle-that began with classical two-dimensional electrophoresis and its advancement during the 1990s, has been revolutionized by a series of tremendous technological developments in mass spectrometry (MS), a core technology. Proteomics is exerting its influence on biological function of genes and genomes in the era (21st century) of functional genomics, and for this reason yeast, bacterial, and mammalian systems are the best examples. Although plant proteomics is still in its infancy, evolving proteomic technologies and the availability of the genome sequences of Arabidopsis thaliana (L.) Heyhn, and rice (Oryza sativa L.), model dicotyledoneous and monocotyledoneous (monocot) species, respectively, are propelling it towards new heights, as evidenced by the rapid spurt in worldwide plant proteome research. Rice, with an immense socio-economic impact on human civilization, is a representative model of cereal food crops, and we consider it as a cornerstone for functional genomics of cereal plants. In this review, we look at the history and the current state of monocot proteomes, including barley, maize, and wheat, with a central focus on rice, which has the most extensive proteomic coverage to date. On one side, we highlight advances in technologies that have generated enormous amount of interest in plant proteomics, and the other side summarizes the achievements made towards establishing proteomes during plant growth & development and challenge to environmental factors, including disease, and for studying genetic relationships. In light of what we have learned from the proteomic journey in rice and other monocots, we finally reveal and assess their impact in our continuous strive towards completion of their full proteomes.
蛋白质组学——对细胞、组织、器官或生物体在生命周期中特定时刻存在的蛋白质进行的系统研究——始于经典的二维电泳及其在20世纪90年代的发展，如今已因质谱（MS）这一核心技术的一系列巨大技术进步而发生了变革。在功能基因组学时代（21世纪），蛋白质组学正在对基因和基因组的生物学功能产生影响，酵母、细菌和哺乳动物系统就是最好的例子。尽管植物蛋白质组学仍处于起步阶段，但不断发展的蛋白质组学技术以及模式双子叶植物拟南芥和单子叶植物水稻基因组序列的可得性，正推动其迈向新高度，全球植物蛋白质组研究的迅速激增就证明了这一点。水稻对人类文明具有巨大的社会经济影响，是谷类粮食作物的代表性模式植物，我们将其视为谷类植物功能基因组学的基石。在本综述中，我们审视单子叶植物蛋白质组的历史和现状，包括大麦、玉米和小麦，重点是水稻，其蛋白质组学覆盖范围是迄今为止最广泛的。一方面，我们强调那些在植物蛋白质组学中引发大量关注的技术进展，另一方面总结在植物生长发育过程中建立蛋白质组以及应对包括疾病在内的环境因素和研究遗传关系方面所取得的成就。鉴于我们从水稻和其他单子叶植物的蛋白质组学历程中学到的知识，我们最终揭示并评估它们在我们持续努力完成其完整蛋白质组过程中的影响。
