Alcaide M, Reina D, Frontera E, Navarrete I
Department of Medicine and Animal Health, Faculty of Veterinary Sciences, University of Extremadura, Spain.
Med Vet Entomol. 2005 Jun;19(2):151-7. doi: 10.1111/j.0269-283X.2005.00555.x.
A serodiagnostic test for the diagnosis of infestation by the sheep nasal bot fly, Oestrus ovis (Linné) was examined. The enzyme-linked immunosorbent assay (ELISA) technique was used to analyze and compare the production of immunoglobulin G (IgG) antibodies against excretory-secretory products (ESP) and crude extract (CE) antigens from all the different larval stages of O. ovis in the sera of 276 adult sheep sampled in summer (n = 135) and winter (n = 141). ESP from first stage larvae was the most sensitive, coating antigen in winter and ESP from second stage larvae during summer. The most specific values were obtained by ESP against L1 in winter and by CE against L3 in summer. These results show that the stage of larval development has a significant impact on the humoral immune response over the course of a season. A significant correlation (P < 0.001) was found between the number of O. ovis larvae and the serum antibody levels using all differents antigens, except L3 CE. In Spain, where a long favourable period exists for the evolution and development of the different stage larvae between March and November, the ELISA test using L1 ESP antigen during winter and L2 ESP antigen in summer may be used for ovine oestrosis immunodiagnosis.
对一种用于诊断绵羊鼻狂蝇（Oestrus ovis，林奈）感染的血清学诊断试验进行了研究。采用酶联免疫吸附测定（ELISA）技术，分析并比较了276只成年绵羊血清中针对绵羊鼻狂蝇不同幼虫阶段排泄分泌产物（ESP）和粗提物（CE）抗原产生的免疫球蛋白G（IgG）抗体，这些绵羊于夏季（n = 135）和冬季（n = 141）采样。来自第一期幼虫的ESP最为敏感，在冬季作为包被抗原，而来自第二期幼虫的ESP在夏季最为敏感。最具特异性的值在冬季由针对L1的ESP获得，在夏季由针对L3的CE获得。这些结果表明，幼虫发育阶段在一个季节的过程中对体液免疫反应有显著影响。使用除L3 CE之外的所有不同抗原时，发现绵羊鼻狂蝇幼虫数量与血清抗体水平之间存在显著相关性（P < 0.001）。在西班牙，3月至11月期间存在不同阶段幼虫发育和生长的长期有利时期，冬季使用L1 ESP抗原以及夏季使用L2 ESP抗原的ELISA试验可用于绵羊狂蝇病的免疫诊断。
