McEwan N R, Abecia L, Regensbogenova M, Adam C L, Findlay P A, Newbold C J
Gut Microbiology and Immunology Division, Rowett Research Institute, Aberdeen, UK.
Lett Appl Microbiol. 2005;41(1):97-101. doi: 10.1111/j.1472-765X.2005.01707.x.
This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed.
Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in long day (16 h light) or short day (8 h light) photoperiods. Bacterial diversity varied according to the daylength conditions where the host animal was housed, as did total volatile fatty acids (VFA) concentrations. No differences associated with daylength were detected in ciliate diversity, branched VFA concentrations or the ruminal ammonia concentrations.
As diets had identical composition, yet voluntary intakes levels were higher during long days, it is proposed that the differences in bacterial populations arise because of the differences in amount of food consumed.
The outcome of this study demonstrated that factors beyond dietary composition must be taken into account when trying to study microbial populations, even in what can be considered a fairly constant environment.
开展此项工作以确定与绵羊饲养日照时长相关的瘤胃微生物种群是否存在差异。
采用变性梯度凝胶电泳（DGGE）研究饲养于长日照（16小时光照）或短日照（8小时光照）光周期下的索艾公羊瘤胃中的纤毛虫和细菌多样性。细菌多样性随宿主动物饲养的日照时长条件而变化，总挥发性脂肪酸（VFA）浓度也是如此。在纤毛虫多样性、支链VFA浓度或瘤胃氨浓度方面未检测到与日照时长相关的差异。
由于日粮组成相同，但长日照期间的自愿采食量水平较高，因此推测细菌种群差异的产生是由于食物摄入量的差异。
本研究结果表明，即使在可被视为相当恒定的环境中，在试图研究微生物种群时，也必须考虑日粮组成以外的因素。
