Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.
J Appl Microbiol. 2009 Dec 1;107(6):1924-34. doi: 10.1111/j.1365-2672.2009.04376.x. Epub 2009 May 12.
To investigate, using culture-independent methods, whether the ruminal bacterial structure, population and fermentation parameters differed between sampling locations and time.
The detectable bacteria and fermentation parameters in the digesta from five locations in the rumen of three cows at three time points were analysed. The PCR-denaturing gradient gel electrophoresis (PCR-DGGE) profiles were similar among digesta samples from five locations (95.4%) and three time points (93.4%) within cows; however, a lower similarity was observed for samples collected from different host animals (85.5%). Rumen pH and concentration of volatile fatty acids (VFA) were affected by time points of sampling relative to feeding.
The detectable bacterial structure in the rumen is highly conserved among different locations and over time, while the quantity of individual bacterial species may change diurnally in response to the feeding.
This study supplies the fundamental understanding of the microbial ecology in the rumen, which is essential for manipulation of ruminal microflora and subsequent improvement in animal production.
使用非培养方法研究不同采样位置和时间对瘤胃细菌结构、种群和发酵参数的影响。
分析了三头奶牛五个不同瘤胃位置三个时间点的消化液中可检测细菌和发酵参数。来自五个位置（95.4%）和三个时间点（93.4%）的消化液样本的 PCR-变性梯度凝胶电泳（PCR-DGGE）图谱相似，但来自不同宿主动物的样本之间的相似性较低（85.5%）。瘤胃 pH 值和挥发性脂肪酸（VFA）浓度受采样时间相对于饲喂时间的影响。
不同位置和时间的瘤胃可检测细菌结构高度保守，而个别细菌种类的数量可能会因昼夜节律而发生变化，以响应采食。
本研究为瘤胃微生物生态学提供了基础认识，这对于瘤胃微生物菌群的操纵和随后的动物生产性能的提高至关重要。
