对猫心丝虫感染可预测的特定抗原条带的免疫印迹分析。
Immunoblot analysis of specific antigen bands predictable for Dirofilaria immitis infection in cats.
作者信息
Hayasaki Mineo, Mori Naoko, Hongo Haruka, Katsuya Akiyo, Song Kun-Ho, Une Satoshi, Ikeda Tadao, Ooi Hong-Kean, Uchida Akihiko
机构信息
Veterinary Clinical Center, School of Veterinary Medicine, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
出版信息
Vet Parasitol. 2005 Aug 10;131(3-4):325-9. doi: 10.1016/j.vetpar.2005.05.007.
Serial sera from four mongrel cats experimentally inoculated with infectious larvae of Dirofilaria immitis were analyzed by immunoblot patterns against a phosphate buffered saline-extract of D. immitis. Antigen-specific protein bands detected indicate that the low molecular weight bands of 36, 32, 22, 19 and 14 kDa, are predictable for positive adult worm infection, suggesting diagnostic usefulness for adult D. immitis infection in cats.
对四只经实验接种犬恶丝虫感染性幼虫的杂种猫的系列血清，采用针对犬恶丝虫磷酸盐缓冲盐水提取物的免疫印迹模式进行分析。检测到的抗原特异性蛋白条带表明，36、32、22、19和14 kDa的低分子量条带可预测成年虫体感染呈阳性，提示其对猫的成年犬恶丝虫感染具有诊断价值。
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