Wang Eryu, Paessler Slobodan, Aguilar Patricia V, Smith Darci R, Coffey Lark L, Kang Wenli, Pfeffer Martin, Olson James, Blair Patrick J, Guevara Carolina, Estrada-Franco Jose, Weaver Scott C
Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA.
Am J Trop Med Hyg. 2005 Jun;72(6):805-10.
An epitope-blocking enzyme-linked immunosorbent assay was developed for the rapid differentiation of serologic responses to enzootic variety IE and ID versus epizootic variety IAB and IC strains of Venezuelan equine encephalitis (VEE) virus. Two monoclonal antibodies that differentially recognize epizootic versus enzootic VEE virus epitopes were used to measure the serotype-specific blocking abilities of antibodies in sera of naturally infected humans, equines, and bovines, as well as in experimentally infected equines. The assay is simple, species-independent, rapid, and sensitive, and will improve surveillance for VEE emergence. It could also be used to determine the epidemic potential of a VEE virus following an intentional introduction for bioterrorism.
开发了一种表位阻断酶联免疫吸附测定法，用于快速区分对委内瑞拉马脑炎（VEE）病毒地方流行性IE和ID变种与流行性IAB和IC毒株的血清学反应。使用两种分别识别流行性和地方流行性VEE病毒表位的单克隆抗体，来测量自然感染的人类、马和牛以及实验感染马的血清中抗体的血清型特异性阻断能力。该测定法简单、不依赖物种、快速且灵敏，将改善对VEE出现的监测。它还可用于确定在因生物恐怖主义而有意引入VEE病毒后该病毒的流行潜力。
