Roehrig J T, Bolin R A
National Center of Infectious Diseases, Centers for Disease Control and Prevention, U.S. Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado 80522, USA.
J Clin Microbiol. 1997 Jul;35(7):1887-90. doi: 10.1128/jcm.35.7.1887-1890.1997.
We used previously characterized murine monoclonal antibodies to develop a panel useful in subtyping Venezuelan equine encephalitis (VEE) viruses by an indirect fluorescent antibody assay. This panel worked well with either prototype VEE viruses or a series of more recent VEE virus isolates. The panel is particularly useful for rapidly differentiating VEE viruses with epidemic-epizootic potential from other endemic varieties of this virus. Using this panel, we identified an antigenic variant of prototype VEE subtype 1E virus currently present in Mexico. This antigenic change in the E2 glycoprotein was confirmed by enzyme-linked immunosorbent assay. Because VEE virus virulence has been associated in part with the E2 glycoprotein, this observed antigenic change in the 1E virus E2 glycoprotein may explain the apparent equine virulence of this unusual VEE 1E virus.
我们使用先前鉴定过的鼠单克隆抗体,开发了一组通过间接荧光抗体检测法对委内瑞拉马脑炎(VEE)病毒进行亚型分型的有用试剂。该试剂组对VEE原型病毒或一系列近期的VEE病毒分离株均效果良好。该试剂组对于快速区分具有流行- epizootic潜力的VEE病毒与该病毒的其他地方性变种特别有用。使用该试剂组,我们鉴定出了目前在墨西哥存在的VEE原型1E亚型病毒的一种抗原变异体。E2糖蛋白中的这种抗原变化通过酶联免疫吸附测定得到了证实。由于VEE病毒的毒力部分与E2糖蛋白相关,在1E病毒E2糖蛋白中观察到的这种抗原变化可能解释了这种不寻常的VEE 1E病毒明显的马毒力。