健康小鼠眼中Toll样受体（TLRs）和核苷酸结合寡聚化结构域蛋白（NODs）的mRNA表达模式。
TLRs and NODs mRNA expression pattern in healthy mouse eye.
作者信息
Rodríguez-Martínez S, Cancino-Díaz M E, Jiménez-Zamudio L, García-Latorre E, Cancino-Díaz J C
机构信息
Laboratorio de Microbiología General, Departamento de Microbiología de la Escuela Nacional de Ciencias Biológicas del Instituto Politecnico Nacional, Carpio y Plan de Ayala, México, México.
出版信息
Br J Ophthalmol. 2005 Jul;89(7):904-10. doi: 10.1136/bjo.2004.056218.
AIMS
To look for TLR and NOD mRNA expression in the healthy eye and in other immune privileged and non-immune privileged mouse organs.
METHODS
Semiquantitative RT-PCR was performed to look for TLR1-9 and NOD1 and NOD2 mRNA expressions in the whole eye, in the anterior (AP) and posterior (PP) portions of the eye, in corneal fibroblasts (CF) and in ovary, brain, testis, heart, lung, and spleen.
RESULTS
All the TLR mRNAs were expressed in the whole eye of Balb/c mice. NIH and C57BL/6 did not express TLR9 and TLR8, respectively. NIH expressed higher levels of TLR1, 2, 3, and 6 than the other strains. C57BL/6 expressed the lowest levels of all TLRs. TLR9, 5, and 4 were the less expressed in all strains. All TLRs were expressed in Balb/c PP and TLR1 was not expressed in AP. In NIH and Balb/c CF the majority of TLRs were overexpressed with LPS. In testis, expression of most TLRs was absent. Non-immune privileged organs expressed most of the TLRs. All the organs expressed NOD1 and NOD2. In PP NOD2 was not expressed.
CONCLUSION
TLRs and NODs are expressed in the eye, and could have an important role in the innate immunity.
目的
探寻Toll样受体（TLR）和核苷酸结合寡聚化结构域蛋白（NOD）mRNA在健康眼部以及其他免疫赦免和非免疫赦免小鼠器官中的表达情况。
方法
采用半定量逆转录聚合酶链反应（RT-PCR），检测TLR1-9、NOD1和NOD2 mRNA在全眼、眼前部（AP）和眼后部（PP）、角膜成纤维细胞（CF）以及卵巢、脑、睾丸、心脏、肺和脾脏中的表达。
结果
所有TLR mRNA均在Balb/c小鼠的全眼中表达。NIH小鼠和C57BL/6小鼠分别不表达TLR9和TLR8。NIH小鼠表达的TLR1、2、3和6水平高于其他品系。C57BL/6小鼠表达的所有TLR水平最低。TLR9、5和4在所有品系中表达较少。所有TLR均在Balb/c小鼠的眼后部表达，而TLR1在眼前部不表达。在NIH小鼠和Balb/c小鼠的角膜成纤维细胞中，大多数TLR在脂多糖（LPS）刺激下过度表达。在睾丸中，大多数TLR不表达。非免疫赦免器官表达大多数TLR。所有器官均表达NOD1和NOD2。在眼后部不表达NOD2。
结论
TLR和NOD在眼中表达，可能在固有免疫中发挥重要作用。
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