Bins Adriaan D, Jorritsma Annelies, Wolkers Monika C, Hung Chien-Fu, Wu T-C, Schumacher Ton N M, Haanen John B A G
Department of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
Nat Med. 2005 Aug;11(8):899-904. doi: 10.1038/nm1264. Epub 2005 Jun 19.
Induction of immunity after DNA vaccination is generally considered a slow process. Here we show that DNA delivery to the skin results in a highly transient pulse of antigen expression. Based on this information, we developed a new rapid and potent intradermal DNA vaccination method. By short-interval intradermal DNA delivery, robust T-cell responses, of a magnitude sufficient to reject established subcutaneous tumors, are generated within 12 d. Moreover, this vaccination strategy confers protecting humoral immunity against influenza A infection within 2 weeks after the start of vaccination. The strength and speed of this newly developed strategy will be beneficial in situations in which immunity is required in the shortest possible time.
DNA疫苗接种后的免疫诱导通常被认为是一个缓慢的过程。在此我们表明，将DNA递送至皮肤会导致抗原表达出现高度短暂的脉冲。基于这一信息，我们开发了一种新的快速有效的皮内DNA疫苗接种方法。通过短间隔皮内DNA递送，在12天内即可产生强大的T细胞反应，其强度足以排斥已形成的皮下肿瘤。此外，这种疫苗接种策略在接种开始后的2周内即可赋予针对甲型流感感染的保护性体液免疫。这种新开发策略的强度和速度在需要尽可能短的时间内产生免疫的情况下将是有益的。
