Büttgenbach S, Wilke R
Technical University of Braunschweig, Institute for Microtechnology, Langer Kamp 8, 38106 Braunschweig, Germany.
Anal Bioanal Chem. 2005 Nov;383(5):733-7. doi: 10.1007/s00216-005-3346-6. Epub 2005 Jun 18.
A microchip-based capillary electrophoresis device supported by a microfluidic network made of poly(dimethylsiloxane), used for measuring target analytes from a continuous sample flow, is presented. The microsystem was fabricated by means of replica molding in combination with standard microfabrication technologies, resulting in microfluidic components and an electrochemical detector. A new hydrodynamic sample injection procedure is introduced, and the maximum number of consecutive measurements that can be made with a poly(dimethylsiloxane) capillary electrophoresis chip with amperometric detection is investigated with respect to reproducibility. The device features a high degree of functional integration, so the benefits associated with miniaturized analysis systems apply to it.
本文介绍了一种基于微芯片的毛细管电泳装置，该装置由聚二甲基硅氧烷制成的微流体网络支撑，用于测量连续样品流中的目标分析物。该微系统通过复制成型结合标准微加工技术制造而成，形成了微流体组件和一个电化学检测器。引入了一种新的流体动力学进样程序，并针对重现性研究了采用安培检测的聚二甲基硅氧烷毛细管电泳芯片能够进行的最大连续测量次数。该装置具有高度的功能集成性，因此适用于小型化分析系统所具备的诸多优点。
