Kurita Ryoji, Yabumoto Norikuni, Niwa Osamu
National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Ibaraki, Japan.
Biosens Bioelectron. 2006 Feb 15;21(8):1649-53. doi: 10.1016/j.bios.2005.07.016. Epub 2005 Aug 29.
We have developed a microfluidic device consisting of a gold film working electrode modified with lactate oxidase and Os-poly(vinylpyridine) mediator containing horseradish peroxide, and reference and counter electrodes in a microflow detection channel separated by a microdialysis membrane from another microflow channel used for sample injection. The dialysis membrane is cellulose with a molecular weight cut off of 10 kDa. We achieved control over a wide recovery rate range of 3-94% because the device is capable of controlling both flow rates in the dual thin-layer channels. We were able to measure the lactate concentration in blood samples within a few minutes without any pretreatment because biomolecules are simultaneously separated by molecular weight and detected in the device. We achieved quantitative and reproducible measurements of the lactate concentration in blood samples, and obtained a relative standard deviation of 1.5% (n = 8). With our device, the lactate concentration in dog whole blood was measured with high stability without any pretreatment.
我们开发了一种微流控装置，该装置由一个金膜工作电极组成，该电极用乳酸氧化酶和含有辣根过氧化物的锇 - 聚乙烯吡啶介体进行了修饰，在微流检测通道中有参比电极和对电极，微流检测通道通过微透析膜与另一个用于样品注入的微流通道隔开。透析膜是截留分子量为10 kDa的纤维素。由于该装置能够控制双薄层通道中的流速，我们实现了在3 - 94%的宽回收率范围内的控制。我们能够在几分钟内无需任何预处理就测量血样中的乳酸浓度，因为生物分子在装置中同时按分子量分离并被检测。我们实现了血样中乳酸浓度的定量和可重复测量，相对标准偏差为1.5%（n = 8）。使用我们的装置，无需任何预处理就以高稳定性测量了犬全血中的乳酸浓度。
