Ikeda Yonosuke, Saito Hiuga, Miura Kin-ichiro, Takagi Jun, Aoki Hatsuo
Institute of Applied Microbiology, University of Tokyo, Tokyo.
J Gen Appl Microbiol. 2004 Dec;50(6):353-62.
With 25 strains belonging to 12 species of the genus Bacillus, the base composition of DNA, the susceptibility to bacteriophages, and the ability to transform Bacillus subtilis strain Marburg were studied. Analyses of phage DNAs were also performed. The results were as follows: (1) The DNA base compositions were not uniform even among strains belonging to one taxonomic species. (2) The DNAs extracted from B. natto, B. megaterium and B. polymyxa could transform genetic traits of B. subtilis Marburg although the frequencies were not equal. (3) The host ranges of some temperate bacteriophages were correlated with the taxonomical data. On these bases, the phylogenetic relatedness of B. subtilis to B. megaterium was discussed.
对属于芽孢杆菌属12个种的25个菌株，研究了其DNA的碱基组成、对噬菌体的敏感性以及转化枯草芽孢杆菌马尔堡菌株的能力。还对噬菌体DNA进行了分析。结果如下：（1）即使在属于一个分类物种的菌株之间，DNA碱基组成也不均匀。（2）从纳豆芽孢杆菌、巨大芽孢杆菌和多粘芽孢杆菌中提取的DNA能够转化枯草芽孢杆菌马尔堡菌株的遗传性状，尽管频率不等。（3）一些温和噬菌体的宿主范围与分类学数据相关。基于这些，讨论了枯草芽孢杆菌与巨大芽孢杆菌的系统发育相关性。
