Anjia Chen, Cunhong Li, Wenhua Gao, Zhide Hu, Xingguo Chen
Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
Biomed Chromatogr. 2005 Sep;19(7):481-7. doi: 10.1002/bmc.464.
A simple, economical and effective non-aqueous capillary electrophoresis separation and detection method was developed for the quantification of deoxyschizandrin and gamma-schizandrin in Schisandra chinensis Baill. and its medicinal preparations for the first time. After optimization of separation conditions, a buffer of 140 mmol/L sodium cholate in methanol was selected for separating the two analytes, but baseline separation of SA and SB in real samples was not obtained. Therefore second-order derivative electropherograms were applied for resolving overlapping peaks. Regression equations revealed good linear relationships (correlation coefficients 0.9975--0.9988) between peak heights in second-order derivative electropherograms and concentrations of the two analytes. The relative standard deviations (RSD) of the migration times and the peak height of the two constituents were in the ranges 0.62--0.79% and 0.25--2.17% (intra-day) and 1.43--2.06 and 4.08--5.72% (inter-day), respectively. The recoveries of the two constituents ranged from 93.2 to 103.0%. The results indicated that baseline separation of the analytes was sometimes hard to obtain in real samples and second-order derivative electropherograms were applicable for the resolution and analysis of overlapping peaks.
首次建立了一种简单、经济且有效的非水毛细管电泳分离检测方法，用于定量五味子及其药用制剂中的五味子醇甲和五味子乙素。在优化分离条件后，选择甲醇中140 mmol/L胆酸钠缓冲液分离这两种分析物，但实际样品中五味子醇甲和五味子乙素未实现基线分离。因此，采用二阶导数电泳图来解析重叠峰。回归方程显示二阶导数电泳图中的峰高与两种分析物浓度之间具有良好的线性关系（相关系数0.9975 - 0.9988）。两种成分迁移时间和峰高的相对标准偏差（RSD）日内分别为0.62 - 0.79%和0.25 - 2.17%，日间分别为1.43 - 2.06和4.08 - 5.72%。两种成分的回收率在93.2%至103.0%之间。结果表明，实际样品中分析物有时难以实现基线分离，二阶导数电泳图适用于重叠峰的解析和分析。
