Bozalioğlu Sema, Ozkan Yeim, Turan Münire, Simşek Bolkan
Biochemistry Laboratory, Faculty of Medicine, Başkent University, 1. Cad. No: 77, 06490 Bahcelievler, Ankara, Turkey.
J Trace Elem Med Biol. 2005;18(3):243-9. doi: 10.1016/j.jtemb.2005.01.003.
Zinc is an essential trace element for many biological functions, including immune functions. The mechanism by which zinc may affect the immune system is certainly multifaceted, due to zinc's widespread action on different enzymes, peptides, transcriptional factors and cytokines involved in the various physiological steps of immune development and reactivity. In this study, prevalence of zinc deficiency and alteration in complement system, immunoglobulins and T cell subsets depending on zinc levels were analyzed in short-term hemodialysis patients and compared with healthy controls. Plasma zinc levels were measured by flame atomic absorption spectrometry. Serum levels of complement C3 and C4, immunoglobulins G (IgG), M (IgM), and A (IgA), and prealbumin were measured by nephelometry depending on antigen-antibody reactions. Percentages of CD4 and CD8+ were calculated using a flow cytometer. Statistically significant decreased zinc levels, especially in the age group > or = 40 years, and increased C4, IgA, IgM, IgG and CD4+ levels were observed in hemodialysis patients. The prevalence of hypozincemia in hemodialysis patients was found to be 40%. A higher CD4+/CD8 ratio was also obtained in patients. We conclude that patients on maintenance hemodialysis for a short time exhibit zinc deficiency and disturbed immune response.
锌是许多生物学功能所必需的微量元素，包括免疫功能。锌影响免疫系统的机制肯定是多方面的，这是因为锌对参与免疫发育和反应性各个生理步骤的不同酶、肽、转录因子和细胞因子具有广泛作用。在本研究中，分析了短期血液透析患者锌缺乏的患病率以及根据锌水平补体系统、免疫球蛋白和T细胞亚群的变化，并与健康对照进行比较。血浆锌水平通过火焰原子吸收光谱法测定。血清补体C3和C4、免疫球蛋白G（IgG）、M（IgM）和A（IgA）以及前白蛋白水平根据抗原-抗体反应通过散射比浊法测定。使用流式细胞仪计算CD4和CD8 +的百分比。在血液透析患者中观察到锌水平有统计学意义的降低，尤其是在年龄≥40岁的年龄组中，同时C4、IgA、IgM、IgG和CD4 +水平升高。血液透析患者低锌血症的患病率为40%。患者的CD4 + / CD8比值也较高。我们得出结论，短期维持性血液透析患者存在锌缺乏和免疫反应紊乱。
