Heck Albert J R, Krijgsveld Jeroen
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
Expert Rev Proteomics. 2004 Oct;1(3):317-26. doi: 10.1586/14789450.1.3.317.
A major aim of present-day proteomics is to study changes in protein expression levels at a global level, ideally monitoring all proteins present in cells or tissue. Mass spectrometry is a well-respected technology in proteomics that is widely used for the identification of proteins. More recently, methodologies have been introduced showing that mass spectrometry can also be used for protein quantification. This article reviews various mass spectrometry-based technologies in quantitative proteomics, highlighting several interesting applications in areas ranging from cell biology to clinical applications.
当今蛋白质组学的一个主要目标是在全球范围内研究蛋白质表达水平的变化，理想情况下是监测细胞或组织中存在的所有蛋白质。质谱分析法是蛋白质组学中一项备受推崇的技术，广泛用于蛋白质的鉴定。最近，已经引入了一些方法，表明质谱分析法也可用于蛋白质定量。本文综述了定量蛋白质组学中各种基于质谱的技术，重点介绍了从细胞生物学到临床应用等领域的一些有趣应用。
