Biological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, Poland.
Int J Mol Sci. 2023 Mar 24;24(7):6129. doi: 10.3390/ijms24076129.
Significant advances in the technological development of mass spectrometry in the field of proteomics and the generation of extremely large amounts of data require a very critical approach to assure the validity of results. Commonly used procedures involved liquid chromatography followed by high-resolution mass spectrometry measurements. Proteomics analysis is used in many fields including the investigation of the metabolism of biologically active substances in organisms. Thus, there is a need to care about the validity of the obtained results. In this work, we proposed a standardized protocol for proteomic analysis using liquid chromatography-high-resolution mass spectrometry, which covers all of these analytical steps to ensure the validity of the results. For this purpose, we explored the requirements of the ISO/IEC 17025:2017 standard as a reference document for quality control in biochemistry research-based mass spectrometry.
在蛋白质组学领域，质谱技术的发展取得了重大进展，产生了大量的数据，这就需要非常严格的方法来保证结果的有效性。常用的方法包括液相色谱法和高分辨率质谱法。蛋白质组学分析被广泛应用于许多领域，包括生物体中生物活性物质代谢的研究。因此，需要关注获得的结果的有效性。在这项工作中，我们提出了一个使用液相色谱-高分辨率质谱进行蛋白质组学分析的标准化方案，涵盖了所有这些分析步骤，以确保结果的有效性。为此，我们探索了 ISO/IEC 17025:2017 标准的要求，将其作为基于质谱的生物化学研究质量控制的参考文件。
