Patel Vibhuti J, Thalassinos Konstantinos, Slade Susan E, Connolly Joanne B, Crombie Andrew, Murrell J Colin, Scrivens James H
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.
J Proteome Res. 2009 Jul;8(7):3752-9. doi: 10.1021/pr900080y.
The proteome of the recently discovered bacterium Methylocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.
利用三种分析和比较蛋白质组学方法对最近发现的西尔维斯特甲基孢囊菌的蛋白质组进行了表征。该生物体已在两种不同的底物上生长，从而能够识别蛋白质表达的差异。已就鉴定出的蛋白质数量、鉴定的可信度、序列覆盖率以及受调控蛋白质的一致性，对使用这些实验方法获得的结果进行了比较。对不同实验的样品制备、仪器使用时间和样品加载要求进行了比较和讨论。还对蛋白质调控结果进行了生物学意义的初步筛选。
