Zhang Sheng, Van Pelt Colleen K
Cornell University, 135 Biotechnology Building, Ithaca, NY 14853, USA.
Expert Rev Proteomics. 2004 Dec;1(4):449-68. doi: 10.1586/14789450.1.4.449.
In the last several years, significant progress has been made in the development of microfluidic-based analytical technologies for proteomic and drug discovery applications. Chip-based nanoelectrospray coupled to a mass spectrometer detector is one of the recently developed analytical microscale technologies. This technology offers unique advantages for automated nanoelectrospray including reduced sample consumption, improved detection sensitivity and enhanced data quality for proteomic studies. This review presents an overview and introduction of recent developments in chip devices coupled to electrospray mass spectrometers including the development of the automated nanoelectrospray ionization chip device for protein characterization. Applications using automated chip-based nanoelectrospray ionization technology in proteomic and bioanalytical studies are also extensively reviewed in the fields of high-throughput protein identification, protein post-translational modification studies, top-down proteomics, biomarker screening by pattern recognition, noncovalent protein-ligand binding for drug discovery and lipid analysis. Additionally, future trends in chip-based nanoelectrospray technology are discussed.
在过去几年中，基于微流控的蛋白质组学和药物发现应用分析技术取得了重大进展。基于芯片的纳米电喷雾与质谱检测器联用是最近开发的分析微尺度技术之一。该技术为自动化纳米电喷雾提供了独特优势，包括减少样品消耗、提高检测灵敏度以及提升蛋白质组学研究的数据质量。本综述概述并介绍了与电喷雾质谱仪联用的芯片装置的最新进展，包括用于蛋白质表征的自动化纳米电喷雾电离芯片装置的开发。还广泛综述了在高通量蛋白质鉴定、蛋白质翻译后修饰研究、自上而下蛋白质组学、模式识别筛选生物标志物、药物发现中非共价蛋白质-配体结合以及脂质分析等领域中使用基于芯片的自动化纳米电喷雾电离技术的应用。此外，还讨论了基于芯片的纳米电喷雾技术的未来趋势。
