Dong Z B, Li S P, Hong M, Zhu Q
National Standard Lab for Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
J Pharm Biomed Anal. 2005 Jul 15;38(4):664-9. doi: 10.1016/j.jpba.2005.02.008. Epub 2005 Apr 9.
The screening and analysis of bioactive components in traditional Chinese medicines (TCMs) is very important not only for the quality control of crude drugs but also for elucidating the therapeutic principle. In this study, a method for screening potential active components from TCMs was developed by using biomembrane extraction and high performance liquid chromatography. Based on the methodology, aqueous extract of Angelica sinensis (WEAS) was used, and four compounds were detected by HPLC in the desorption eluate of red cell membrane extraction for WEAS. The compounds were identified as ferulic acid, ligustilide, senkyunolide H and senkyunolide I based on their UV, MS and NMR spectra. Actually, ferulic acid and ligustilide are considered as major active components in Angelica sinensis. Therefore, this method may be applied to predict the potential bioactivities of multiple compounds in TCMs simultaneously.
中药生物活性成分的筛选与分析不仅对中药材的质量控制非常重要，而且对于阐明其治疗原理也很重要。在本研究中，开发了一种利用生物膜萃取和高效液相色谱法从中药中筛选潜在活性成分的方法。基于该方法，使用了当归水提取物（WEAS），通过高效液相色谱法在WEAS红细胞膜萃取的解吸洗脱液中检测到了四种化合物。根据它们的紫外光谱、质谱和核磁共振光谱，这些化合物被鉴定为阿魏酸、藁本内酯、川芎内酯H和川芎内酯I。实际上，阿魏酸和藁本内酯被认为是当归中的主要活性成分。因此，该方法可用于同时预测中药中多种化合物的潜在生物活性。
