Takeda Seiji, Sbagyo Agus, Sakoda Yoshihiro, Ishii Atsushi, Sawamura Makoto, Sueoka Kazuhisa, Kida Hiroshi, Mukasa Koichi, Matsumoto Kazuhiko
Graduate School of Engineering, Hokkaido University, Kita 13 Nishi 8 Kita-ku, Sapporo 060-8628, Japan.
Biosens Bioelectron. 2005 Jul 15;21(1):201-5. doi: 10.1016/j.bios.2004.08.030.
Carbon nanotube sensors detected anti-hemagglutinin binding to immobilized hemagglutinins. An ultra-sensitive detection method for antibodies or antigens in serum is required. Hemagglutinins were immobilized on the reverse side of a carbon nanotube, thereby producing a source and a drain. Electrode pads covered each edge of the nanotube. The I-V curves between the source and the drain were measured after incubation of anti-hemagglutinins with immobilized hemagglutinins in a buffered solution on the reverse side of the nanotube. The sensitivity of the CNT sensor was higher than that of an ELISA system. This method constitutes a new tool to analyze interaction among biomolecules on a substrate.
碳纳米管传感器检测到抗血凝素与固定化血凝素的结合。需要一种用于检测血清中抗体或抗原的超灵敏检测方法。将血凝素固定在碳纳米管的背面，从而产生一个源极和一个漏极。电极焊盘覆盖在纳米管的每个边缘。在纳米管背面的缓冲溶液中，将抗血凝素与固定化血凝素孵育后，测量源极和漏极之间的I-V曲线。碳纳米管传感器的灵敏度高于酶联免疫吸附测定（ELISA）系统。该方法构成了一种分析底物上生物分子间相互作用的新工具。
