Withey G D, Lazareck A D, Tzolov M B, Yin A, Aich P, Yeh J I, Xu J M
Division of Engineering, Brown University, Providence, RI 02912, USA.
Biosens Bioelectron. 2006 Feb 15;21(8):1560-5. doi: 10.1016/j.bios.2005.07.014. Epub 2005 Aug 29.
We report on a highly ordered array of carbon nanotubes (CNTs) that serves as a universally direct nanoelectrode interface for redox proteins and provides an efficient conduit for electron transfer. The site-selective, covalent docking of the enzyme glucose oxidase (GO(x)) on the CNT tips is found to have a marked effect on enhancing electron transfer properties, as measured by cyclic voltammetry. A unimolecular electron transfer rate of 1500 s(-1) has been measured for this system, a value exceeding the rate of oxygen reduction by glucose oxidase. Furthermore, the redox enzyme-CNT array conjugate can be utilized as a quantitative, substrate-specific biosensor.
我们报道了一种高度有序的碳纳米管（CNT）阵列，它作为氧化还原蛋白的通用直接纳米电极界面，并为电子转移提供了高效通道。通过循环伏安法测量发现，葡萄糖氧化酶（GO(x)）在碳纳米管尖端的位点选择性共价对接对增强电子转移特性有显著影响。该系统测得的单分子电子转移速率为1500 s(-1)，这一数值超过了葡萄糖氧化酶还原氧气的速率。此外，氧化还原酶-碳纳米管阵列共轭物可作为一种定量的、底物特异性生物传感器。
