Daubenfeld Thorsten, Hossann Martin, Trommer Wolfgang E, Niedner-Schatteburg Gereon
Laboratoire des Mécanismes Réactionnels, UMR 7651, Ecole Polytechnique, 91128 Palaiseau Cedex, France.
Biochem Biophys Res Commun. 2005 Aug 5;333(3):984-9. doi: 10.1016/j.bbrc.2005.06.008.
The amino acid sequence and the glycosylation motif of the ribosome inactivating protein (RIP) gelonin are identified by Fourier transform ion cyclotron resonance mass spectrometry. Intact gelonin as isolated from the seeds of Gelonium multiflorum consists of at least three different post-translational modified forms: analysis of gelonin peptides as obtained by proteolytic digestion is consistent with the amino acid sequence published by Nolan et al. High resolution mass determination established a glycosylation pattern of GlcNAc2Man(3-5)Xyl. N189 was identified as glycosylation site. The proposed glycan structure is consistent with a standard plant N-glycosylation pattern as found in other RIP. Based on these results we suggest that gelonin is located in the vacuole of Gelonium multiflorum seeds.
通过傅里叶变换离子回旋共振质谱法鉴定了核糖体失活蛋白（RIP）米托蒽醌的氨基酸序列和糖基化基序。从多花美登木种子中分离得到的完整米托蒽醌至少由三种不同的翻译后修饰形式组成：对通过蛋白水解消化获得的米托蒽醌肽段的分析与Nolan等人发表的氨基酸序列一致。高分辨率质量测定确定了GlcNAc2Man(3-5)Xyl的糖基化模式。N189被鉴定为糖基化位点。所提出的聚糖结构与其他RIP中发现的标准植物N-糖基化模式一致。基于这些结果，我们认为米托蒽醌位于多花美登木种子的液泡中。
