Krause Kirsten, Kilbienski Isabell, Mulisch Maria, Rödiger Anja, Schäfer Anke, Krupinska Karin
Institute of Botany, University of Kiel, Olshausenstr. 40, D-24098 Kiel, Germany.
FEBS Lett. 2005 Jul 4;579(17):3707-12. doi: 10.1016/j.febslet.2005.05.059.
Arabidopsis thaliana contains three genes with high homology to potato p24 which was described as a member of the Whirly family of nuclear transcriptional activators. Computer-based analysis revealed that all Arabidopsis Whirly (Why) proteins contain targeting sequences for either plastids or mitochondria. The functionality of these sequences was demonstrated by in vitro import assays into isolated organelles. Transient expression of GFP fusion proteins in protoplasts and onion epidermal cells confirmed the localisation of these proteins in plastids or mitochondria, respectively. The possession of organellar targeting sequences seems to be conserved among Why proteins of higher plant species, including potato p24.
拟南芥含有三个与马铃薯p24高度同源的基因，马铃薯p24被描述为核转录激活因子Whirly家族的一个成员。基于计算机的分析表明，所有拟南芥Whirly（Why）蛋白都含有针对质体或线粒体的靶向序列。这些序列的功能通过体外导入分离细胞器的实验得以证明。GFP融合蛋白在原生质体和洋葱表皮细胞中的瞬时表达分别证实了这些蛋白在质体或线粒体中的定位。在包括马铃薯p24在内的高等植物物种的Why蛋白中，似乎都保留了细胞器靶向序列。
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