Funes Soledad, Gerdes Lars, Inaba Masami, Soll Jürgen, Herrmann Johannes M
Institut für Physiologische Chemie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5, 81377 München, Germany.
FEBS Lett. 2004 Jul 2;569(1-3):89-93. doi: 10.1016/j.febslet.2004.05.055.
The Arabidopsis thaliana protein ARTEMIS is an integral component of the chloroplast inner envelope required for chloroplast division. It contains a domain of significant homology to members of the Alb3/Oxa1/YidC protein family. Here, we show that upon expression in yeast mitochondria, ARTEMIS can partially take over the function of yeast Oxa1 in the insertion and assembly of mitochondrial membrane proteins. This identifies ARTEMIS as a functional member of the Alb3/Oxa1/YidC protein family and suggests the existence of a novel protein sorting pathway in chloroplasts which integrates polypeptides from the stroma into the inner envelope by an evolutionary conserved process.
拟南芥蛋白ARTEMIS是叶绿体分裂所需的叶绿体内膜的一个组成成分。它含有一个与Alb3/Oxa1/YidC蛋白家族成员具有显著同源性的结构域。在这里，我们表明，在酵母线粒体中表达时，ARTEMIS可以部分接管酵母Oxa1在线粒体膜蛋白插入和组装中的功能。这确定ARTEMIS是Alb3/Oxa1/YidC蛋白家族的一个功能成员，并表明叶绿体中存在一种新的蛋白质分选途径，该途径通过一个进化保守的过程将来自基质的多肽整合到内膜中。
