Sherratt Michael J, Bax Daniel V, Chaudhry Shazia S, Hodson Nigel, Lu Jian R, Saravanapavan Priya, Kielty Cay M
Division of Laboratory and Regenerative Medicine, The Medical School, Stopford Building, The University of Manchester, UK.
Biomaterials. 2005 Dec;26(34):7192-206. doi: 10.1016/j.biomaterials.2005.05.010.
In addition to mediating cell signalling events, native extracellular matrix (ECM) assemblies interact with other ECM components, act as reservoirs for soluble signalling molecules and perform structural roles. The potential of native ECM assemblies in the manufacture of biomimetic materials has not been fully exploited due, in part, to the effects of substrate interactions on their morphology. We have previously demonstrated that the ECM components, fibrillin and type VI collagen microfibrils, exhibit substrate dependent morphologies on chemically and topographically variable heterogeneous surfaces. Using both cleaning and coating approaches on silicon wafers and glass coverslips we have produced chemically homogeneous, topographically similar substrates which cover a large amphiphilic range. Extremes of substrate amphiphilicity induced morphological changes in periodicity, curvature and lateral spreading which may mask binding sites or disrupt domain structure. Biological functionality, as assayed by the ability to support cell spreading, was significantly reduced for fibrillin microfibrils adsorbed on highly hydrophilic substrates (contact angle 20.7 degrees) compared with less hydrophilic (contact angle 38.3 degrees) and hydrophobic (contact angle 92.8 degrees) substrates. With an appropriate choice of surface chemistry, multifunctional ECM assemblies retain their native morphology and biological functionality.
除了介导细胞信号传导事件外，天然细胞外基质（ECM）组件还与其他ECM成分相互作用，充当可溶性信号分子的储存库并发挥结构作用。天然ECM组件在制造仿生材料方面的潜力尚未得到充分利用，部分原因是底物相互作用对其形态的影响。我们之前已经证明，ECM成分原纤维蛋白和VI型胶原微纤维在化学和地形可变的异质表面上呈现出底物依赖性形态。通过在硅片和玻璃盖玻片上使用清洁和涂层方法，我们制备了化学性质均匀、地形相似的底物，这些底物涵盖了较大的两亲性范围。底物两亲性的极端情况会导致周期性、曲率和横向扩展的形态变化，这可能会掩盖结合位点或破坏结构域结构。与亲水性较低（接触角38.3度）和疏水性（接触角92.8度）的底物相比，吸附在高亲水性底物（接触角20.7度）上的原纤维蛋白微纤维，通过支持细胞铺展能力测定的生物学功能显著降低。通过适当选择表面化学，多功能ECM组件可以保留其天然形态和生物学功能。
