从一起与牡蛎相关的肠胃炎暴发中鉴定出多种诺如病毒基因型。

Multiple norovirus genotypes characterised from an oyster-associated outbreak of gastroenteritis.

作者信息

Gallimore Chris I, Cheesbrough John S, Lamden Kenneth, Bingham Chris, Gray Jim J

机构信息

Enteric, Respiratory and Neurological Virus Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency Centre for Infections, Colindale, London, NW9 5HT, United Kingdom.

出版信息

Int J Food Microbiol. 2005 Sep 15;103(3):323-30. doi: 10.1016/j.ijfoodmicro.2005.02.003.

DOI:10.1016/j.ijfoodmicro.2005.02.003

PMID:15967530

Abstract

The diversity of norovirus (NV) genotypes was investigated in persons who were ill with acute gastroenteritis associated with the consumption of oysters. Initial results from a commercial enzyme immunoassay (EIA) indicated a mixed NV genogroup I (GI) and II (GII) outbreak. A reverse-transcriptase (RT)-PCR for NVs was applied to nucleic acid extracted from faecal specimens collected from symptomatic cases. Using primers that amplified contiguous sequences in the ORF1/2 region of the NV genome and a hemi-nested PCR derived from this assay, three different GII and two GI NV genotypes were detected and the strains were characterised by DNA sequencing. Using this approach a recombinant NV genotype, rGII-3a (recombinant Harrow/Mexico) the predominant strain identified in several symptomatic cases from the outbreak, was detected and characterised. No other gastroenteric viruses, including rotavirus, astrovirus, sapovirus and adenovirus 40/41 were detected by RT-PCR and PCR.

摘要

对因食用牡蛎而患急性肠胃炎的患者的诺如病毒（NV）基因型多样性进行了调查。商业酶免疫测定（EIA）的初步结果表明，这是一起NV基因I组（GI）和II组（GII）混合暴发事件。将针对NV的逆转录酶（RT）-PCR应用于从有症状病例采集的粪便标本中提取的核酸。使用在NV基因组ORF1/2区域扩增连续序列的引物以及由此检测法衍生的半巢式PCR，检测到三种不同的GII和两种GI NV基因型，并通过DNA测序对菌株进行了表征。采用这种方法，检测并鉴定出一种重组NV基因型rGII-3a（重组哈罗/墨西哥株），它是此次暴发中几例有症状病例中鉴定出的主要菌株。通过RT-PCR和PCR未检测到其他胃肠病毒，包括轮状病毒、星状病毒、札幌病毒和腺病毒40/41。