Jin Miao, Sun Jun-ling, Chang Zhao-rui, Li Hui-ying, Liu Na, Zhang Qing, Cui Shu-xian, Zhang Jing, Wang Zi-jun, Duan Zhao-jun
Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052.
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 May;31(5):549-53.
To acknowledge the epidemiology of gastroenteritis outbreaks caused by noroviruses and their genotypes.
Epidemiologic data and specimens were collected from 19 gastroenteritis outbreaks. 201 specimens were detected for norovirus, rotavirus, astrovirus, adenovirus and sapovirus by RT-PCR methods and PCR products were sequenced. Sequence alignment and phylogenetic analysis were performed by Clustal X 1.83 and MEGA 4.0 programs.
Noroviruses were one of the most predominant pathogens causing viral gastroenteritis outbreaks (12 of 19 outbreaks, accounting for 63.2%). Variant GII-4/2006b was the predominant strain responsible for 11 of the 12 NV-associated outbreaks. Other genotypes would include GII-17, GII-6 and GII-3. The NV-associated gastroenteritis outbreaks occurred mainly in winter and spring between December 2006 and April 2007. These gastroenteritis outbreaks caused by noroviruses would involve all age groups in various locations. Meantime, 2 out of 12 outbreaks were caused by norovirus or other viruses. In addition, multiple viruses and multiple genotypes of noroviruses were found in the same outbreak.
Noroviruses were one of the most major pathogens causing gastroenteritis outbreaks while GII-4/2006b variant was identified as the predominant strain in China.
了解由诺如病毒及其基因型引起的胃肠炎暴发的流行病学特征。
收集19起胃肠炎暴发事件的流行病学数据和标本。采用逆转录聚合酶链反应（RT-PCR）方法对201份标本检测诺如病毒、轮状病毒、星状病毒、腺病毒和札如病毒，并对PCR产物进行测序。使用Clustal X 1.83和MEGA 4.0软件进行序列比对和系统发育分析。
诺如病毒是引起病毒性胃肠炎暴发的最主要病原体之一（19起暴发事件中有12起，占63.2%）。变异型GII-4/2006b是12起与诺如病毒相关暴发事件中11起的主要毒株。其他基因型包括GII-17、GII-6和GII-3。与诺如病毒相关的胃肠炎暴发主要发生在2006年12月至2007年4月的冬春季节。这些由诺如病毒引起的胃肠炎暴发涉及不同地点的所有年龄组。同时，12起暴发事件中有2起是由诺如病毒或其他病毒引起的。此外，在同一暴发事件中发现了多种病毒和多种基因型的诺如病毒。
诺如病毒是引起胃肠炎暴发的最主要病原体之一，而GII-4/2006b变异型被确定为中国的主要毒株。
