Biswas Subhajit, Jackson Philippa, Shannon Rebecca, Dulwich Katherine, Sukla Soumi, Dixon Ronald A
University of Lincoln, School of Life Sciences, Brayford Pool, Lincoln, Lincolnshire, United Kingdom.
University of Lincoln, School of Life Sciences, Brayford Pool, Lincoln, Lincolnshire, United Kingdom.
Braz J Microbiol. 2018 Apr-Jun;49(2):279-284. doi: 10.1016/j.bjm.2017.06.006. Epub 2017 Oct 13.
This molecular study is the first report, to the best of our knowledge, on identification of norovirus, NoV GII.4 Sydney 2012 variants, from blue mussels collected from UK coastal waters. Blue mussels (three pooled samples from twelve mussels) collected during the 2013 summer months from UK coastal sites were screened by RT-PCR assays. PCR products of RdRP gene for noroviruses were purified, sequenced and subjected to phylogenetic analysis. All the samples tested positive for NoVs. Sequencing revealed that the NoV partial RdRP gene sequences from two pooled samples clustered with the pandemic "GII.4 Sydney variants" whilst the other pooled sample clustered with the NoV GII.2 variants. This molecular study indicated mussel contamination with pathogenic NoVs even during mid-summer in UK coastal waters which posed potential risk of NoV outbreaks irrespective of season. As the detection of Sydney 2012 NoV from our preliminary study of natural coastal mussels interestingly corroborated with NoV outbreaks in nearby areas during the same period, it emphasizes the importance of environmental surveillance work for forecast of high risk zones of NoV outbreaks.
据我们所知，这项分子研究是首次关于从英国沿海水域采集的蓝贻贝中鉴定诺如病毒（NoV GII.4悉尼2012变种）的报告。通过RT-PCR检测对2013年夏季从英国沿海地点采集的蓝贻贝（来自12只贻贝的3个混合样本）进行了筛查。对诺如病毒RdRP基因的PCR产物进行了纯化、测序并进行了系统发育分析。所有样本诺如病毒检测均呈阳性。测序显示，来自两个混合样本的诺如病毒部分RdRP基因序列与大流行的“GII.4悉尼变种”聚类，而另一个混合样本与诺如病毒GII.2变种聚类。这项分子研究表明，即使在英国沿海水域的仲夏期间，贻贝也受到致病性诺如病毒的污染，这带来了诺如病毒爆发的潜在风险，且与季节无关。由于我们对天然沿海贻贝的初步研究中检测到2012年悉尼诺如病毒，有趣的是这与同期附近地区的诺如病毒爆发情况相符，这强调了环境监测工作对预测诺如病毒爆发高风险区域的重要性。
