Epstein Joshua, Yaccoby Shmuel
Myeloma Institute for Research and Therapy, University of Arkansas for Medical Science, Little Rock, AK, USA.
Methods Mol Med. 2005;113:183-90. doi: 10.1385/1-59259-916-8:183.
The severe combined immune deficient human (SCID-hu) myeloma model is the only available model in which primary myeloma cells grow in vivo in a human bone marrow micro environment. A SCID mouse receives an implanted human fetal bone into which myeloma cells are directly injected. Through interaction with the human bone marrow microenvironment, the myeloma cells induce typical myeloma manifestations in the SCID host, such as the appearance of M protein in the serum, and changes in the implanted human bone, which often result in osteolysis of the human bone. The model provides the only platform for in vivo investigation of the biology and therapy of primary human myeloma in a human microenvironment. This chapter describes in detail all the steps necessary to establish this model and evaluate its success.
严重联合免疫缺陷人类（SCID-hu）骨髓瘤模型是唯一可用的模型，在该模型中，原发性骨髓瘤细胞可在人类骨髓微环境中于体内生长。一只SCID小鼠接受植入的人类胎儿骨，然后将骨髓瘤细胞直接注射到其中。通过与人类骨髓微环境相互作用，骨髓瘤细胞在SCID宿主中诱发典型的骨髓瘤表现，例如血清中出现M蛋白，以及植入的人类骨发生变化，这常常导致人类骨的骨质溶解。该模型为在人类微环境中对原发性人类骨髓瘤的生物学特性和治疗方法进行体内研究提供了唯一的平台。本章详细描述了建立该模型并评估其是否成功所需的所有步骤。
