Galm Oliver, Herman James G
Medizinische Klinik IV, Universitaetsklinikum Aachen, Aachen, Germany.
Methods Mol Med. 2005;113:279-91. doi: 10.1385/1-59259-916-8:279.
Methylation-specific polymerase chain reaction (MSP) is a method that can rapidly assess the methylation status of virtually any group of CpG sites within a CpG island, independent of the use of methylation-sensitive restriction enzymes. This assay entails the initial modification of DNA by sodium bisulfite, converting all unmethylated cytosines to uracils but leaving the methylated cytosines unchanged, followed by subsequent amplification with primers specific for methylated vs unmethylated DNA. The great sensitivity of this technique allows qualitative methylation analysis from DNA obtained not only from fresh frozen tissues, peripheral blood, bone marrow, or body fluids but also from paraffin-embedded samples. It is a rapid and cost-effective method that does not require radioactive reagents and can be used for the analysis of a large number of clinical samples.
甲基化特异性聚合酶链反应（MSP）是一种能够快速评估CpG岛内几乎任何一组CpG位点甲基化状态的方法，无需使用甲基化敏感限制酶。该检测方法首先用亚硫酸氢钠对DNA进行修饰，将所有未甲基化的胞嘧啶转化为尿嘧啶，但甲基化的胞嘧啶保持不变，随后用针对甲基化与未甲基化DNA的特异性引物进行扩增。这项技术的高灵敏度使得不仅可以对从新鲜冷冻组织、外周血、骨髓或体液中获取的DNA进行定性甲基化分析，还能用于石蜡包埋样本。它是一种快速且经济高效的方法，不需要放射性试剂，可用于大量临床样本的分析。
