Galons Jean-Philippe, Lope-Piedrafita Silvia, Divijak Joseph L, Corum Curt, Gillies Robert J, Trouard Theodore P
Department of Radiology, University of Arizona, Tucson, Arizona 85724, USA.
Magn Reson Med. 2005 Jul;54(1):79-86. doi: 10.1002/mrm.20565.
The complexity of biologic tissues, with multiple compartments each with its own diffusion and relaxation properties, requires complex formalisms to model water signal in most magnetic resonance imaging or magnetic resonance spectroscopy experiments. In this article, we describe a magnetic susceptibility-induced shift in the resonance frequency of extracellular water by the introduction of a gadolinium contrast agent to medium perfusing a hollow fiber bioreactor. The frequency shift of the extracellular water (+185 Hz at 9.4 T) uncovers the intracellular water and allows direct measurement of motional and relaxation properties of the intracellular space. The proposed method provides a unique tool for understanding the mechanisms underlining diffusion and relaxation in the intracellular space.
生物组织具有多个隔室，每个隔室都有其自身的扩散和弛豫特性，这种复杂性使得在大多数磁共振成像或磁共振波谱实验中，需要复杂的形式体系来对水信号进行建模。在本文中，我们描述了通过向灌注中空纤维生物反应器的介质中引入钆造影剂，细胞外水的共振频率发生了由磁化率引起的偏移。细胞外水的频率偏移（在9.4T时为+185Hz）揭示了细胞内水，并允许直接测量细胞内空间的运动和弛豫特性。所提出的方法为理解细胞内空间中扩散和弛豫的潜在机制提供了一个独特的工具。
