Yu Rong-Min, Song Yong-Bo, Zhang Hui, Ye Wen-Cai, Zhang Yin-Lin, Yao Xin-Sheng
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Sheng Wu Gong Cheng Xue Bao. 2003 May;19(3):372-5.
It was clearly demonstrated that T-DNA of Agrobacterium tumefeciens Ti plasmid was integrated into the cells of crown gall in our experiment. This paper reported the influences of some kinds of physical-chemistry factors on the growth of crown gall of Panax quinquefolium and the production of its main active compounds--ginsenoside Re and ginsensoside Rg1. The results showed that White medium was the best one for ginsensoside Rg1 accumulation (0.095%) among the six media, but ginsensoside Re accumulation (0.194%) was the highest on the MS medium; The highest contents of ginsensoside Re (0.147%) and ginsensoside Rg1 (0.061%) were on the culture 36d and 32d after innoculum respectively; The optimum pH was 5.6 for ginsensoside Rg1 synthesis(0.054%), and 5.8 for ginsensoside Re synthesis(0. 184% ); The contents of ginsensoside Re and ginsensoside Rg1 was the highest in the inoculum of 4 g and 2 g/flask (FW) respectively. The result also indicated that the concentration of inositol in 0.05 g/L could obviously promote ginsensoside Re synthesis (0.182%), and in 0.30 g/L for ginsensoside Rg1 (0.055%).
在我们的实验中已清楚表明，根癌农杆菌Ti质粒的T-DNA整合到了冠瘿瘤细胞中。本文报道了某些理化因素对西洋参冠瘿瘤生长及其主要活性成分——人参皂苷Re和人参皂苷Rg1产生的影响。结果表明，在六种培养基中，White培养基对人参皂苷Rg1积累（0.095%）而言是最佳的，但在MS培养基上人参皂苷Re积累（0.194%）最高；人参皂苷Re（0.147%）和人参皂苷Rg1（0.061%）的最高含量分别在接种后36天和32天的培养物中；人参皂苷Rg1合成（0.054%）的最适pH为5.6，人参皂苷Re合成（0.184%）的最适pH为5.8；人参皂苷Re和人参皂苷Rg1的含量分别在接种量为4 g和2 g/瓶（鲜重）时最高。结果还表明，0.05 g/L的肌醇浓度可明显促进人参皂苷Re合成（0.182%），0.30 g/L的肌醇浓度对人参皂苷Rg1（0.055%）有促进作用。
