[三七悬浮培养中人参皂苷生物合成的动力学]

[Dynamics of ginsenoside biosynthesis in suspension culture of Panax notoginseng].

作者信息

Wang Yan, Wu Yang-Yang, Ge Feng, Yu Hong

机构信息

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.

Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, China.

出版信息

Zhong Yao Cai. 2013 Jul;36(7):1059-62.

PMID:24417138

Abstract

OBJECTIVE

To study the dynamics of ginsenoside biosynthesis in suspension culture of Panax notoginseng.

METHODS

The calli of Panax notoginseng were suspended in liquid medium for 40 d, and the changes of ginsenosides were determined by HPLC method.

RESULTS

Cell growth was slow in suspension culture, but the contents of six ginsenosides varied with different culture time. The contents of four ginsenosides Rg1, Re, Rh1 and Rb1 showed the increases from about 3.63 to 9.72 mg/g, 2.34 to 7.02 mg/g, 0.42 to 2.37 mg/g and 0.24 to 1.86 mg/g, respectively during 25 and 30 days of the culture. Rh2 and F1 had no obvious change. Such results indicated that the protopanaxatriol saponions (Rg1, Re and Rh1) increased significantly during the growth phase.

CONCLUSION

When the callus is cultured for 25 days, the maximum yield of total saponins content is up to 20.58 mg/g,mainly of the protopanaxatriol saponins.

摘要

目的

研究三七悬浮培养中人参皂苷生物合成的动态变化。

方法

将三七愈伤组织在液体培养基中悬浮培养40天，采用高效液相色谱法测定人参皂苷的变化。

结果

悬浮培养中细胞生长缓慢，但六种人参皂苷的含量随培养时间不同而变化。在培养25至30天期间，四种人参皂苷Rg1、Re、Rh1和Rb1的含量分别从约3.63毫克/克增加到9.72毫克/克、2.34毫克/克增加到7.02毫克/克、0.42毫克/克增加到2.37毫克/克和0.24毫克/克增加到1.86毫克/克。Rh2和F1无明显变化。这些结果表明，原人参三醇皂苷（Rg1、Re和Rh1）在生长阶段显著增加。