发根农杆菌介导的紫锥菊遗传转化及转化培养物中生物活性成分分析

Genetic transformation of Echinacea purpurea with Agrobacterium rhizogenes and bioactive ingredient analysis in transformed cultures.

作者信息

Wang Bochu, Zhang Guangqiu, Zhu Liancai, Chen Lingxi, Zhang Yali

机构信息

Key Lab for Biomechanics and Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, PR China.

出版信息

Colloids Surf B Biointerfaces. 2006 Nov 1;53(1):101-4. doi: 10.1016/j.colsurfb.2006.08.003. Epub 2006 Aug 17.

DOI:10.1016/j.colsurfb.2006.08.003

PMID:16982176

Abstract

A method of the transformed hairy roots cultures of Echinacea purpurea was established by infecting different types of explants with three type strains of Agrobacterium rhizogenes (A4, R1601 and R1000) in this paper. We obtained that the transformed percentage of E. purpurea leaves with A4, R1601 and R1000 were 80%, 60%, 40%, respectively and that of E. purpurea leafstalks were 10%, 30%, 45%, respectively. The contents of polysaccharides and phenolic compounds were measured in transformed hairy roots and non-transformed roots after 2 months in culture. For transformed hairy roots, the contents of polysaccharides and phenolic compounds were 236.0 and 18.9 mg g(-1) DW, respectively. While the contents of polysaccharides and phenolic compounds in non-transformed roots were 161.5 and 33.3 mg g(-1) DW, respectively.

摘要

本文通过用三种发根农杆菌（A4、R1601和R1000）菌株感染不同类型的外植体，建立了紫锥菊转化毛状根培养方法。我们发现，用A4、R1601和R1000转化紫锥菊叶片的转化率分别为80%、60%、40%，转化紫锥菊叶柄的转化率分别为10%、30%、45%。培养2个月后，测定了转化毛状根和未转化根中多糖和酚类化合物的含量。对于转化毛状根，多糖和酚类化合物的含量分别为236.0和18.9 mg g(-1) DW。而未转化根中多糖和酚类化合物的含量分别为161.5和33.3 mg g(-1) DW。