Oliveira Diana M, Gouveia Joao Jose S, Diniz Nilo B, Pacheco Ana Carolina L, Vasconcelos Elton Jose R, Diniz Michely C, Viana Daniel A, Ferreira Thiago D, Albuquerque Marianna C, Fortier Daniel C, Maia Allan R S, Costa Luis A C, Melo João Osmar P, da Silva Maria Cristina, Walter Cezar A, Faria Jose O, Tome Adriana R, Gomes Marcos Jose N, Oliveira Sonia M P, Araújo-Filho Raimundo, Costa Raimundo B, Maggioni Rodrigo
Núcleo de Genômica e Bioinformática, Faculdade de Veterinária, Universidade Estadual do Ceara (UECE), Campus do Itaperi, Fortaleza, Ceara, Brazil.
OMICS. 2005 Summer;9(2):173-93. doi: 10.1089/omi.2005.9.173.
The trypanosomatid flagellar apparatus contains conventional and unique features, whose roles in infectivity are still enigmatic. Although the flagellum and the flagellar pocket are critical organelles responsible for all vesicular trafficking between the cytoplasm and cell surface, still very little is known about their roles in pathogenesis and how molecules get to and from the flagellar pocket. The ongoing analysis of the genome sequences and proteome profiles of Leishmania major and L infantum, Trypanosoma cruzi, T. brucei, and T. gambiensi ( www.genedb.org ), coupled with our own work on L. chagasi (as part of the Brazilian Northeast Genome Program- www.progene.ufpe.br ), prompted us to scrutinize flagellar genes and proteins of Leishmania spp. promastigotes that could be virulence factors in leishmaniasis. We have identified some overlooked parasite factors such as the MNUDC-1 (a protein involved in nuclear development and genomic fusion) and SQS (an enzyme of sterol biosynthesis), among the described flagellar gene families. A database concerning the results of this work, as well as of other studies of Leishmania and its organelles, is available at http://nugen.lcc.uece.br/LPGate . It will serve as a convenient bioinformatics resource on genomics and pathology of the etiological agents of leishmaniasis.
锥虫鞭毛器兼具传统特征和独特特征，其在感染性方面的作用仍不明确。尽管鞭毛和鞭毛袋是负责细胞质与细胞表面之间所有囊泡运输的关键细胞器，但人们对它们在发病机制中的作用以及分子如何进出鞭毛袋仍知之甚少。对硕大利什曼原虫、婴儿利什曼原虫、克氏锥虫、布氏锥虫和冈比亚锥虫的基因组序列和蛋白质组图谱的持续分析（www.genedb.org），以及我们自己对恰加斯利什曼原虫的研究工作（作为巴西东北基因组计划的一部分——www.progene.ufpe.br），促使我们仔细研究利什曼原虫前鞭毛体中可能是利什曼病致病因素的鞭毛基因和蛋白质。在已描述的鞭毛基因家族中，我们已经鉴定出一些被忽视的寄生虫因素，如MNUDC-1（一种参与核发育和基因组融合的蛋白质）和SQS（一种甾醇生物合成酶）。有关这项工作以及利什曼原虫及其细胞器的其他研究结果的数据库可在http://nugen.lcc.uece.br/LPGate获取。它将作为利什曼病病原体基因组学和病理学方面便捷的生物信息学资源。
