Kéry V, Orlovská M, Stancíková M, Risko M, Zlnay D
Research Institute of Rheumatic Diseases, Piestany, Czechoslovakia.
Clin Chem. 1992 Jun;38(6):841-6.
We used Alcian Blue (AB) and dimethylmethylene blue (DMB) methods to measure glycosaminoglycan (GAG) excretion in the first morning urine specimens of patients with osteoarthritis (OA), ankylosing spondylitis (AS), and rheumatoid arthritis (RA) in different stages of disease. By the AB method, urinary GAG excretion in patients with RA was not different from healthy control subjects. However, the DMB method showed significant differences (in milligrams of GAG per gram of creatinine) for OA (median 25.4, range 14.3-44.0, P less than 0.01, n = 23) and RA patients (median 33.0; range 10.0-147.6; P less than 0.001, n = 63) in comparison with unaffected individuals (median 20.2; range 8.9-41.4, n = 38). We noted a significant difference in urinary GAG excretion between RA and OA patients (P less than 0.01) and between RA and AS (P less than 0.01) patients. The DMB method was further investigated by clinical decision analysis. The DMB method is simple and rapid and may be useful in diagnosing RA by distinguishing between RA and OA or AS.
我们采用阿尔辛蓝（AB）法和二甲基亚甲基蓝（DMB）法，对骨关节炎（OA）、强直性脊柱炎（AS）和类风湿关节炎（RA）不同疾病阶段患者晨尿样本中的糖胺聚糖（GAG）排泄情况进行了检测。采用AB法时，RA患者的尿GAG排泄量与健康对照者并无差异。然而，DMB法显示，与未受影响个体（中位数20.2；范围8.9 - 41.4，n = 38）相比，OA患者（中位数25.4，范围14.3 - 44.0，P < 0.01，n = 23）和RA患者（中位数33.0；范围10.0 - 147.6；P < 0.001，n = 63）的GAG排泄量（每克肌酐中GAG的毫克数）存在显著差异。我们注意到，RA与OA患者之间（P < 0.01）以及RA与AS患者之间（P < 0.01）的尿GAG排泄量存在显著差异。通过临床决策分析对DMB法进行了进一步研究。DMB法简单快速，在区分RA与OA或AS以诊断RA方面可能有用。
