Department of Medical Biochemistry, Ministry of Health, Ankara Training and Research Hospital, Ankara, Turkey.
Clin Chem Lab Med. 2013 Feb;51(2):347-50. doi: 10.1515/cclm-2012-0471.
The study aim was to compare the performance of three different methods used for determining urinary glycosaminoglycans (GAG) levels in spot and 24-h urine samples.
Performance characteristics were studied for cetylpyridinium chloride (CPC), and manual and automated dimethylmethylene blue (DMB) methods.
For automated DMB method, within-run precisions were 9.10% and 1.98%, and between-day precisions were 13.0% and 5.81% in low- and high-urine pools, respectively. The method was linear up to 100 mg/L of GAG concentration. The detection limit of the method was 0.71 mg/L. Mean recovery was 95.7%.
The automated DMB method was found to give better performance characteristics than cetylpyridinium chloride (CPC) and manual DMB methods. It is a fast, cheap, simple and reliable method and can be applied in many diseases in which GAG is used as a screening test.
本研究旨在比较三种不同方法在检测尿糖胺聚糖（GAG）水平时的性能，这三种方法分别用于检测点尿样和 24 小时尿样。
研究了 cetylpyridinium chloride（CPC）、手动和自动二甲亚甲基蓝（DMB）方法的性能特征。
对于自动 DMB 方法，在低尿池和高尿池中的日内精密度分别为 9.10%和 1.98%，日间精密度分别为 13.0%和 5.81%。该方法在 GAG 浓度达 100mg/L 时呈线性。方法的检测限为 0.71mg/L。平均回收率为 95.7%。
与 cetylpyridinium chloride（CPC）和手动 DMB 方法相比，自动 DMB 方法具有更好的性能特征。它是一种快速、廉价、简单和可靠的方法，可应用于许多将 GAG 作为筛选试验的疾病中。
