不同筛查方法在尿氨基己糖苷类检测中的性能比较。
Performance of different screening methods for the determination of urinary glycosaminoclycans.
机构信息
Department of Medical Biochemistry, Ministry of Health, Ankara Training and Research Hospital, Ankara, Turkey.
出版信息
Clin Chem Lab Med. 2013 Feb;51(2):347-50. doi: 10.1515/cclm-2012-0471.
BACKGROUND
The study aim was to compare the performance of three different methods used for determining urinary glycosaminoglycans (GAG) levels in spot and 24-h urine samples.
METHODS
Performance characteristics were studied for cetylpyridinium chloride (CPC), and manual and automated dimethylmethylene blue (DMB) methods.
RESULTS
For automated DMB method, within-run precisions were 9.10% and 1.98%, and between-day precisions were 13.0% and 5.81% in low- and high-urine pools, respectively. The method was linear up to 100 mg/L of GAG concentration. The detection limit of the method was 0.71 mg/L. Mean recovery was 95.7%.
CONCLUSIONS
The automated DMB method was found to give better performance characteristics than cetylpyridinium chloride (CPC) and manual DMB methods. It is a fast, cheap, simple and reliable method and can be applied in many diseases in which GAG is used as a screening test.
背景
本研究旨在比较三种不同方法在检测尿糖胺聚糖（GAG）水平时的性能，这三种方法分别用于检测点尿样和 24 小时尿样。
方法
研究了 cetylpyridinium chloride（CPC）、手动和自动二甲亚甲基蓝（DMB）方法的性能特征。
结果
对于自动 DMB 方法，在低尿池和高尿池中的日内精密度分别为 9.10%和 1.98%，日间精密度分别为 13.0%和 5.81%。该方法在 GAG 浓度达 100mg/L 时呈线性。方法的检测限为 0.71mg/L。平均回收率为 95.7%。
结论
与 cetylpyridinium chloride（CPC）和手动 DMB 方法相比，自动 DMB 方法具有更好的性能特征。它是一种快速、廉价、简单和可靠的方法，可应用于许多将 GAG 作为筛选试验的疾病中。
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