Heinonen M V, Purhonen A K, Miettinen P, Pääkkönen M, Pirinen E, Alhava E, Akerman K, Herzig K H
Department of Biotechnology/Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, 70211 Kuopio, Finland.
Regul Pept. 2005 Aug 15;130(1-2):7-13. doi: 10.1016/j.regpep.2005.05.003.
Maintenance of human energy homeostasis is regulated by a complex network. Peptides secreted from the gastrointestinal tract (GI) are signaling to the brain and other organs initiating or terminating food intake and energy expenditure. In the present study we investigated basal plasma levels of apelin, orexin-A, and leptin in morbid obese patients. In addition, we measured in a subgroup of these patients in the same individual orexin-A and leptin plasma levels one year after gastric banding surgery.
Basal plasma values were determined in obese patients (BMI=48+/-1 kg/m2n=32) after an overnight fast and compared to healthy, normal weighted (BMI=22+/-2 kg/m2n=12) controls. In addition, blood samples were collected in a subgroup of patients (BMI=48+/-1 kg/m2n=8) the day before surgery and 1 year after the operation. Apelin, orexin-A, and leptin levels were analysed using ELISAs.
One year after the operation obese patients significantly lost weight (from 48+/-2 kg/m2 to 39+/-2 kg/m2; p<0,001). Apelin, orexin-A and leptin levels in obese patients were significantly higher compared to control individuals (736+/-50 pg/ml vs. 174+/-14 pg/ml, p<0.0001; 75.3+/-24.1 pg/ml vs. 0.8+/-0.4 pg/ml, p<0.0001; 79.0+/-2.4 ng/ml vs. 5.8+/-0.8 ng/ml, p<0.0001, respectively). Apelin and leptin plasma concentrations also correlated significantly with BMI (r=0.769, p<0.0001; r=0.778; p<0.0001, respectively), while orexin-A correlation was rather weak (r=0.335, p<0.03). No difference between pre- and post-operative orexin-A levels was observed, while leptin plasma levels significantly decreased from 45.1+/-5.4 ng/ml to 27.3+/-6.0 ng/ml (p=0.015).
Apelin, orexin-A, and leptin plasma levels correlated positively with the BMI. One year after gastric banding with significant loss in BMI basal plasma levels of leptin decreased, while orexin-A remained unchanged.
人体能量平衡的维持受复杂网络调控。胃肠道（GI）分泌的肽向大脑和其他器官发出信号，启动或终止食物摄入及能量消耗。在本研究中，我们调查了病态肥胖患者血浆中阿片肽、食欲素 - A和瘦素的基础水平。此外，我们对这些患者中的一个亚组在胃束带手术后一年测量了同一患者体内食欲素 - A和瘦素的血浆水平。
在肥胖患者（BMI = 48±1 kg/m²，n = 32）禁食过夜后测定基础血浆值，并与健康、体重正常（BMI = 22±2 kg/m²，n = 12）的对照组进行比较。此外，在一组患者（BMI = 48±1 kg/m²，n = 8）手术前一天和术后1年采集血样。使用酶联免疫吸附测定法（ELISA）分析阿片肽、食欲素 - A和瘦素水平。
术后一年肥胖患者体重显著减轻（从48±2 kg/m²降至39±2 kg/m²；p < 0.001）。肥胖患者的阿片肽、食欲素 - A和瘦素水平显著高于对照组个体（分别为736±50 pg/ml对174±14 pg/ml，p < 0.0001；75.3±24.1 pg/ml对0.8±0.4 pg/ml，p < 0.0001；79.0±2.4 ng/ml对5.8±0.8 ng/ml，p < 0.0001）。阿片肽和瘦素的血浆浓度也与BMI显著相关（分别为r = 0.769，p < 0.0001；r = 0.778；p < 0.0001），而食欲素 - A的相关性较弱（r = 0.335，p < 0.03）。未观察到术前和术后食欲素 - A水平的差异，而瘦素血浆水平从45.1±5.4 ng/ml显著降至27.3±6.0 ng/ml（p = 0.015）。
阿片肽、食欲素 - A和瘦素的血浆水平与BMI呈正相关。胃束带手术后一年，随着BMI显著下降，瘦素的基础血浆水平降低，而食欲素 - A保持不变。
