Tsuchikane Yuki, Fujii Tadashi, Ito Motomi, Sekimoto Hiroyuki
Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902 Japan.
Plant Cell Physiol. 2005 Sep;46(9):1472-6. doi: 10.1093/pcp/pci158. Epub 2005 Jun 20.
The sex pheromone protoplast release-inducing protein (PR-IP) inducer and a sexual cell division-inducing pheromone-minus (SCD-IP-minus) that mediates the sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale (C. psl) complex were investigated in this study. Recombinant PR-IP inducer produced by yeast cells was prepared and assayed for production of PR-IP and induction of SCD. Both biological activities were observed after treating mating-type plus (mt+) cells with the recombinant pheromone. SCD was induced by exposure to a lower concentration of the same pheromone and by a shorter treatment period with the pheromone than was production of PR-IP. This indicates that the previously characterized PR-IP inducer has both PR-IP-inducing and SCD-inducing activities with mt+ cells, although the inducing mechanisms of the two pheromones differ.
本研究对性信息素原生质体释放诱导蛋白（PR-IP）诱导剂和一种介导异宗配合的纤细新月藻-条带新月藻-滨海新月藻（C. psl）复合体有性生殖的性细胞分裂诱导信息素缺失型（SCD-IP-minus）进行了研究。制备了酵母细胞产生的重组PR-IP诱导剂，并检测其PR-IP的产生及SCD的诱导情况。用重组信息素处理交配型正（mt+）细胞后，观察到了这两种生物学活性。与PR-IP的产生相比，较低浓度的相同信息素暴露以及较短的信息素处理时间即可诱导SCD。这表明，尽管两种信息素的诱导机制不同，但先前鉴定的PR-IP诱导剂对mt+细胞同时具有PR-IP诱导和SCD诱导活性。
