Sekimoto Hiroyuki, Tanabe Yoichi, Tsuchikane Yuki, Shirosaki Hiroshi, Fukuda Hiroo, Demura Taku, Ito Motomi
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo 112-8681, Japan.
Plant Physiol. 2006 May;141(1):271-9. doi: 10.1104/pp.106.078048. Epub 2006 Mar 24.
The desmid Closterium peracerosum-strigosum-littorale complex, which is the closest unicellular sister to land plants, is the best characterized of the charophycean green algae with respect to the process of sexual reproduction. To elucidate the molecular mechanism of intercellular communication during sexual reproduction, we created a normalized cDNA library from mixed cells of the sexual and the vegetative phases and generated a cDNA microarray. In total, 3,236 expressed sequence tags, which were classified into 1,615 nonredundant groups, were generated for cDNA microarray construction. Candidate genes for key factors involved in fertilization, such as those that encode putative receptor-like protein kinase, leucine-rich-repeat receptor-like protein, and sex pheromone homologs, were up-regulated during sexual reproduction and/or by the addition of the purified sex pheromones, and the expression patterns of these genes were confirmed by quantitative real-time polymerase chain reaction analysis. This first transcriptome profile of Closterium will provide critical clues as to the mechanism and evolution of intercellular communication between the egg and sperm cells of land plants.
新月藻（Closterium peracerosum-strigosum-littorale）复合体是陆地植物最接近的单细胞姐妹，在有性生殖过程方面，是轮藻门绿藻中特征最明确的。为了阐明有性生殖期间细胞间通讯的分子机制，我们从有性和营养阶段的混合细胞中创建了一个标准化的cDNA文库，并生成了一个cDNA微阵列。总共生成了3236个表达序列标签，这些标签被分类为1615个非冗余组，用于构建cDNA微阵列。参与受精的关键因子的候选基因，如那些编码假定的类受体蛋白激酶、富含亮氨酸重复序列的类受体蛋白和性信息素同源物的基因，在有性生殖期间和/或通过添加纯化的性信息素而上调，并且这些基因的表达模式通过定量实时聚合酶链反应分析得到证实。新月藻的这第一个转录组图谱将为陆地植物卵细胞和精细胞之间细胞间通讯的机制和进化提供关键线索。
