Zourob Mohammed, Mohr Stephan, Fielden Peter R, Goddard Nicholas J
School of Chemical Engineering and Analytical Science, The University of Manchester, P. O. Box 88, Sackville Street, Manchester, M60 1QD, UK.
Lab Chip. 2005 Jul;5(7):772-7. doi: 10.1039/b502361k. Epub 2005 May 19.
An integrated, disposable, dye clad leaky waveguide (DCLW) device has been fabricated and tested for both refractive index and fluorescence detection in mu-TAS applications. The chip comprises the required flow geometry and optical coupling elements in a robust device that is relatively simple and inexpensive to fabricate. Disposable DCLW chips were fabricated at room temperature by spin-coating both the dye and silica sol-gel waveguiding layers on a polymer substrate which contained injection moulded grating coupler. These devices have been designed to increase the interaction of the evanescent field light at the channel wall and with the sample in the channel. The DCLW device has been used to detect changes in the refractive index of different percentages of glycerol solutions and to detect low concentrations down to 10(-12) M fluorescein using a grating coupler.
一种集成的、一次性使用的、染料包覆泄漏波导（DCLW）器件已被制造出来，并在微全分析系统（μ-TAS）应用中进行了折射率和荧光检测测试。该芯片在一个坚固的器件中包含所需的流动几何结构和光耦合元件，制造相对简单且成本低廉。一次性DCLW芯片是在室温下通过在包含注塑光栅耦合器的聚合物基板上旋涂染料和二氧化硅溶胶 - 凝胶波导层制成的。这些器件旨在增强通道壁处倏逝场光与通道内样品之间的相互作用。DCLW器件已被用于检测不同百分比甘油溶液的折射率变化，并使用光栅耦合器检测低至10^(-12) M的荧光素低浓度。
