犬嗜吞噬细胞无形体感染：利用聚合酶链反应鉴定病原体

Anaplasma phagocytophilum infection in a dog: identifying the causative agent using PCR.

作者信息

Kirtz G, Meli M, Leidinger E, Ludwig P, Thum D, Czettel B, Kölbl S, Lutz H

机构信息

INVITRO, Laboratory for Veterinary Diagnosis, A-1030 Vienna, Austria.

出版信息

J Small Anim Pract. 2005 Jun;46(6):300-3. doi: 10.1111/j.1748-5827.2005.tb00325.x.

DOI:10.1111/j.1748-5827.2005.tb00325.x

PMID:15971902

Abstract

A diagnosis of Anaplasma phagocytophilum infection was confirmed in a two-year-old male golden retriever displaying few clinical and haematological abnormalities. This was achieved by demonstrating ehrlichial organisms in circulating neutrophils, by indirect immunofluorescence assay using A phagocytophilum as an antigen, and by detecting DNA specific for the 16S rRNA gene of granulocytic Anaplasma by PCR. After treatment with doxycycline for 10 days the dog showed improvement and the laboratory values returned to normal.

摘要

一只两岁雄性金毛猎犬被确诊感染嗜吞噬细胞无形体，该犬几乎没有临床和血液学异常表现。这一诊断是通过在循环中性粒细胞中证实埃立克体生物、使用嗜吞噬细胞无形体作为抗原进行间接免疫荧光测定以及通过聚合酶链反应检测粒细胞无形体16S rRNA基因的特异性DNA来实现的。用强力霉素治疗10天后，该犬病情好转，实验室检查值恢复正常。