Mao Ping, Wang Cai-Xia, Lin Xiu-Mei, Du Qing-Hua
Department of Hematology, The First Guangzhou Municipal People Hospital Affiliated to Guangzhou Medical College, Guangzhou 510180, China. bai
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Jun;13(3):422-8.
This study was aimed to explore the role of human fetal bone marrow stromal cells (FBMSC) in combination with exogenous cytokines in supporting the in vitro expansion of cord blood mononuclear cells and the expression of CXCR4(+) and CD49d(+) in CD34(+) cells. Mononuclear cells (MNC) separated from cord blood (CB) were cultured in a serum-free support culture system with FBMSC or exogenous cytokines or both of them. On day 0, 6, 10 and 14, total cells were counted, CD34(+), CD34(+)CXCR4(+) and CD34(+)CD49d(+) cells were quantitated by FACS, and hematopoietic progenitor cells were assessed by semisolid culture assay. The results showed that after culturing for 14 days, CD34(+) cells, CD34(+)CXCR4(+) cells, CD34(+) CD49d(+) cells and colony forming unit (CFU) were significantly increased (P < 0.05). Compared with other groups, expansion multiple of CD34(+), CD34(+)CXCR4(+), CD34(+)CD49d(+) cells and CFU were higher than that in FBMSC and cytokine group (P < 0.05). It is concluded that the culture system used in this study can not only support the expansion of CB MNCs but also increase the number of hematopoietic stem and progenitor cells which has chemokine and adhesion capacity. This culture system may be a feasible way for in vitro culture of cord blood cells.
本研究旨在探讨人胎儿骨髓基质细胞（FBMSC）联合外源性细胞因子在支持脐血单个核细胞体外扩增以及CD34(+)细胞中CXCR4(+)和CD49d(+)表达方面的作用。从脐血（CB）中分离出的单个核细胞（MNC）在无血清支持培养体系中与FBMSC或外源性细胞因子或两者一起培养。在第0、6、10和14天，计数总细胞数，通过流式细胞术定量CD34(+)、CD34(+)CXCR4(+)和CD34(+)CD49d(+)细胞，并通过半固体培养试验评估造血祖细胞。结果显示，培养14天后，CD34(+)细胞、CD34(+)CXCR4(+)细胞、CD34(+)CD49d(+)细胞和集落形成单位（CFU）显著增加（P<0.05）。与其他组相比，CD34(+)、CD34(+)CXCR4(+)、CD34(+)CD49d(+)细胞和CFU的扩增倍数高于FBMSC和细胞因子组（P<0.05）。结论是，本研究中使用的培养体系不仅可以支持脐血单个核细胞的扩增，还可以增加具有趋化因子和黏附能力的造血干细胞和祖细胞的数量。该培养体系可能是脐血细胞体外培养的一种可行方法。
