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[Rh弱D及Del样本检测的研究]

[Study on detection of samples of Rh-weak D and Del].

作者信息

Wang Xiao-Zhu, Lan Jiong-Cai, Wu Xu-Hua, Zhou Hua-You, Liu Wei-Jia, Liu Dan, Qi Cun-Sheng, Zeng Feng-Qin, Du Ke-Qing

机构信息

Laboratory of Blood Transfusion, Qinhuangdao Blood Center, Qinhuangdao 066001, China.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Jun;13(3):509-11.

Abstract

To study the detection of weak D and Del from samples initially screened RhD(-), RhD phenotype was initially screened by routine serological test, out of which weak D phenotype was detected by indirect antiglobulin test (IAT) and Del phenotype was detected by chloroform-trichloroethylene absorption-elution test. The results showed that 56 samples were RhD(-) confirmed by routine serology test, which were screened out of 26 200 donors, among them 5 samples were typed as weak D by IAT and 9 cases samples were typed as Del by absorption-elution test. In conclusion, the samples which typed as RhD(-) by routine serological test must be identified by IAT and chloroform-trchloroethylene absorption test is order to detect weak D and Del phenotype. It is important for clinical transfusion safely.

摘要

为研究从最初筛查为RhD阴性的样本中检测弱D和Del,最初通过常规血清学试验筛查RhD血型,其中通过间接抗球蛋白试验(IAT)检测弱D血型,通过氯仿 - 三氯乙烯吸收 - 洗脱试验检测Del血型。结果显示,26200名献血者中经常规血清学试验确认56份样本为RhD阴性,其中5份样本经IAT鉴定为弱D型,9份样本经吸收 - 洗脱试验鉴定为Del型。总之,对于经常规血清学试验鉴定为RhD阴性的样本,必须通过IAT和氯仿 - 三氯乙烯吸收试验进行鉴定,以检测弱D和Del血型。这对临床输血安全很重要。

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